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Anya
Vs
Mayday

Gwen
Vs
Silk

Winners fight each other then the winner fights Jessica Drew
100% healed between rounds

Who is the top Spider (female?)
Fight in 616 NY

Mayday beats Anya, then whoever the winner of Gwen vs Silk is (not sure there). Then Jessica Drew.

May's both really experienced, and has some unexpected powers that give her a nice edge. I think she has the strongest spider sense, but importantly, she also has the ability to make surfaces sticky, which can nullify the agility of the others at a bad time.

"IMO" I think that it will be a toss up between Mayday and Jessica Drew. The way I see it Mayday will beat Anya, and even though her experience is only implied, I'd give the win to Spider-Gwen as Silk has not as much experience. In a fight between Mayday and Spider-Gwen I'd lean towards Mayday, and that may be simply be bias on my part. Then it would be a split between May and Jessica.

I'd vote for Silk