I don't believe that Parker would need prep to defeat Piotr. Piotr obviously has the durability advantage, and the strength, but Parker bring speed, agility, precognitive powers, and a percentage of strength to the table. Could enough uninterruptible hits hurt Piotr? This should really be the question here. I for one believe that it would be possible. He did defeat Firelord with enough punches, even though the fight was PIS. However how much PIS was involved?
1. Firelord could have ended the battle with a large enough blast which would have killed Spiderman.
2. His flight speed should have also given him a huge advantage, but Piotr doesn't use blast, and can not fly.
Those two things made the battle PIS, but the blows that put Firelord down and out wasn't PIS. Piotr is likely more durable to physical attacks than Firelord, because Parker would most likely break his hands punching him directly, but what would happen if he repeatedly webbed Piotr up, and slammed him into the concrete environment. I believe that he could eventually win this way. So what I'm basically saying is that it wouldn't be physically impossible for Spiderman to beat the hell out of the much slower, less agile, one trick pony that Piotr actually is.
Colossus wins 9/10 if he manages to land a blow.
Spiderman wins 8/10 if he manages to evade.
Why no? Strength isn't everything, and people need to recall that Spiderman took hits from a Phoenix empowered Colossus and wasn't KO'd. You can't just argue that strength, and durability wins out every time. Spiderman has strength, agility, speed, resources (webs and tech), clinging abilities, and precognitive powers, that he has regularly used. He wins IMO but it would take a long time.
Colossus will be as fresh as a daisy. How long can Spidey jump away? 5 mins? 10? An hour? Three hours? At the end of the time period, he will be close to collapse, for as long as it takes. He will start making mistakes.
Meanwhile, every punch he lands on Colossus won't do squat. And a hundred won't do squat either. They don't add up, he's not the Flash. If a child taps me, it won't hurt me. A hundred won't hurt me, nor a thousand.
It's PIS if it happens once or twice, but Spiderman does these things often. Sorry but your refusal to acknowledge him based on being weaker in this scenario is weak. Spiderman wins, because he never ever has to engage Piotr on his terms. Peter isn't a dummy man character, but instead is one of the more brilliant characters around. For him to jump into Piotr's face like a noob, would be PIS. This is truly his fight to lose. If they fought it out in a closet, then sure, Piotr wins by a landslide.