Nah. First, Punisher outfits himself with a wrist-mounted cutting torch, a heavy-caliber rifle with armor piercing rounds, a remotely detonated shaped charge, and a bunch of grenades.
He walks up to the Brood and stands there passively as the Drones swarm around him. As one grabs him and hefts him over its back, intending to bring him back to the hive, he slaps a shaped charge under the thing's thorax.
The broodlings return Frank to the hive and web him up in a corner. They place a Brood Egg in front of him, so the baby form can hatch. Frank waits patiently until the Drone he tagged moves a safe distance away (optimally, he'll wait until it approaches the Queen). Then he presses the button on that detonator and takes out about 1/3rd of the hive right there.
Easily freeing himself with the cutting torch, Frank pulls out the rifle and starts unloading. If he's lucky, that blast took out the Queen, but if not, the drones are still going to be in panic and chaos for a while. Frank finds some corner of the hive where he can settle behind cover and digs in, and after that its his game. Any broodling that wanders into his sites gets a round between the eyes. Grenades deal with any massed charges.
The Brood probably retreat after most of their warriors and drones are dead. The Queen probably won't risk fighting herself, but now Frank has her and the last survivors on the run....
Uhh you did say "The Brood" right? Not Aliens? Cause if its the Brood Frank Castle is dead in like 3 seconds. yes the brood are very similar in the way they look, except that the brood are far more intellignent and come in a veriety of flavors, from flying to burring to hulk armor plated monsters. Frank Castle has no real chance agianst them as the first wound he takes startrs to turn him into one of them.